Everton midfielder James Garner and Brighton goalkeeper Jason Steele are the two uncapped players named by Thomas Tuchel in an expanded 35-man England squad.
It is Tuchel's penultimate squad before he makes his selection for the 2026 World Cup.
The extended squad sees recalls for Manchester United duo Harry Maguire and Kobbie Mainoo, while AC Milan defender Fikayo Tomori is back for the first time since November 2018.
Leeds striker Dominic Calvert-Lewin returns after more than five years since his last call-up and Dominic Solanke makes his first squad since October 2024.
But there is no place in Tuchel's squad for Real Madrid full-back Trent Alexander-Arnold or Bournemouth midfielder Alex Scott.
Chelsea's Reece James and Trevoh Chalobah, who were named in the last squad, miss out through injury.
The Three Lions continue their World Cup preparations with friendlies at Wembley Stadium against Uruguay on 27 March and Japan on 31 March.