Thomas Tuchel said England's upcoming camp is the "last opportunity for players to compete for their ticket" to the World Cup this summer as he named an expanded 35-man squad.
It included two uncapped players in Everton midfielder James Garner and Brighton goalkeeper Jason Steele.
The extended squad saw recalls for Manchester United duo Harry Maguire and Kobbie Mainoo, while AC Milan defender Fikayo Tomori is back for the first time since October 2023.
Leeds striker Dominic Calvert-Lewin returns after more than five years since his last call-up and Dominic Solanke makes his first squad since October 2024.
But there is no place in Tuchel's squad for Real Madrid full-back Trent Alexander-Arnold, Bournemouth midfielder Alex Scott or Aston Villa forward Ollie Watkins.
Chelsea's Reece James and Trevoh Chalobah, who were named in the last squad, miss out through injury.
The Three Lions continue their World Cup preparations with friendlies at Wembley Stadium against Uruguay on 27 March and Japan on 31 March.
"It's our the last camp [before the World Cup]. The last opportunity to impress and the last opportunity for players to compete for their ticket," Tuchel said.
Tuchel said to "get the best out of this camp" a decision was made to split it into two camps, with 11 players joining the squad after the Uruguay game.
The 11 players are: Dean Henderson, Dan Burn, Marc Guehi, Ezri Konsa, Nico O'Reilly, Elliot Anderson, Declan Rice, Morgan Rogers, Anthony Gordon, Harry Kane and Bukayo Saka.
"These guys have played 3,500 minutes, some 4,000 minutes. More important than the pure number of minutes is some of these guys have played more minutes than the whole of last season," Tuchel said.
"All of these players have contributed in September, October and November. They have credit with me. To give them a break mentally and physically, we will benefit from it."
Teams must submit their final World Cup squads by Saturday, 30 May.